|
KMID : 0811720000040000021
|
|
Korean Journal of Physiology & Pharmacology
2000 Volume.4 No. 0 p.21 ~ p.0
|
|
|
Effects of IκB and Bcl-2 overexpression on cell death in mouse cortical collecting duct cells
|
|
Song Jin-Mi
Lee Ryang-Hwa
Park Mi-Yong
Hwang Suk-Mi
Kang Soo-Kyung
|
|
|
Abstract
|
|
|
Recent data have implicated nuclear factor κB (NFκB) and Bcl-2 in the prevention of apoptosis in various cell lines. However, it has been also reported that NF-κB plays an important role in inflammatory responses in renal epithelial cells and that effect of Bcl-2 in necrotic cell death is not clear. We investigated the effect of specific inhibition of NFκB and overexpression of Bcl-2 on oxidant or cisplatin-induced injury of renal collecting duct cells. M-1 cells, which were derived from outer cortical collecting duct, were stably transfected with the dominant negative mutant inhibitor of NFκB (NFκBαM) and Bcl-2. IκBαM significantly decreased oxidant-induced injury, but had ono significant effects on cisplatin-induced necrotic cell death. However, IκBαM sensitized cells to apoptosis induced by cisplatin or serum deprivation. Overexpression of Bcl-2 inhibited the apoptotic cell death induced by serum diprivation, treatment of cisplatin and contact inhibition, but did not affect oxidant-induced necrotic cell injury in collecting duct cells. These data show that activation of NFκB mediates oxidants-induced necrotic injury, but inhibits apoptotic cell death in renal collecting duct cells.
Source: Korean Journal of Physiology & Pharmacology.2000 Oct;4(Suppl):S18-S18
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|